Solvent Extraction and Chemical Characterization of Papaya Seeds Oil

Jiveri B 1* , Muoki A 2 and Kwanyi M 1

To cite this article : Jiveri, B., Muoki, A., & Kwanyi, M. (2018). Solvent Extraction and Chemical Characterization of Papaya Seeds Oil. Int J Agr Life Sci, 4(4), 248-251. doi: 10.22573/spg.ijals.018.s12200092

To link to this article : https://doi.org/10.22573/spg.ijals.018.s12200092

Copyright : © 2018 Jiveri B et al . This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the Original work is properly cited.

Data Availability Statement : All relevant data are within the paper and its Supporting Information files. Funding : The author(s) received no specific funding for this work. Competing Interests : The authors have declared that no competing interests exist.

© 2018 The Author(s). Published by S k y f o x Published online: 31 Dec 2018.

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RESEARCH ARTICLE

Solvent Extraction and Chemical Characterization of Papaya Seeds Oil Jiveri B

1*, Muoki A 2 and Kwanyi M 1

1. Department of Botany, Maseno University. P.O BOX 333-40105 Maseno, Kenya.

2. Department of Institutional Management, Siaya institute of Technology, P.O BOX 1087-40600, Siaya, Kenya *Author to whom correspondence should be addressed; E-Mail: jkhajezo@gmail.com

Received: Nov 2018 / Accepted: Nov 2018/ Published: Dec 2018

INTRODUCTION

Papaya ( Carica papaya L.) is a plant that grows wild in many parts of the tropics. The seeds of papaya fruits are generally discarded. However, in order to make a more efficient use of papaya, it is worth investigating the use of the seeds as a source of oil. A compound present in crushed papaya seed that is believed to have activity against helminthes intestinal parasites, benzyl isothiocynate, has been shown to have an effect on vascular contraction using a canine carotid artery in vitro model (Wilson and Kwan, 2002). Papaya seed oil utilized in high amount such oils could lead to reduced risk of coronary heart disease. In addition, high oleic oil has sufficiently stability be used in demanding application such as frying. Area of spray oil for snacks, crackers, cereal dried fruit, bakery products where the oil is used to maintain product quality and to increase palatability. Papaya seed oil can be considered as high oleic oil and hence viewed as a healthy alternative to many other vegetable oils (Corbett, et al., 2003).

The extraction and use of vegetable oils has for centuries played an important role in the manufacture of a large number of industrial products and food items. Currently, two main processes for the extraction of oil from seeds are of industrial importance: the hydraulic process and further purification and the chemical process using organic solvents (McGlone et al ., 1986). This latter process for extracting oil from seeds, although giving high oil extraction recovery, requires expensive capital investment and operational costs and causes undesirable effects on the quality of end products because of the high temperatures used in the process (Christensen, et al ., 1991). There is also an older process of aqueous extraction that is advantageous, because it presents no risk of fire or explosion, is nontoxic and the mild processing ensures high quality products (Dominguez et al ., 1995). The operation is also more flexible with less initial investment and operational costs. The main disadvantages, as compared to conventional technologies, are lower efficiency of oil extraction and the reduction of product stability, leading to easier microbial contamination. Aqueous extraction, on the other hand, yields not more than 35% of the oil content of the seed (Tano-Debrah and Ohta 1994).

Considering their widespread applications, these processes thus contribute to major losses of fats and oils in the world’s food production system. This is an issue worth considering as efforts are intensified to increase fat and oil production to meet the quickly growing global demands (Tano-Debrah and Ohta 1995a). With rising value for oils and with demands for better oil quality, coupled with several years of unfavorable climate conditions in growing regions, there has been a noticeable increase in trials using enzymes for processing a wide variety of oil sources including palm, olive, soybean, rapeseed, sunflower seed, cottonseed, corn germ and groundnut (Godfrey and Reichelt 1983). The use of enzymes shows some improvement in the yield of oil, together with a reduction in the acid development and oxidation of the oil during further processing and storage. Also, there is a reduction in undesirable side products and in waste treatment costs (Dominguez et al ., 1994). Much research has been done on enzyme-assisted oil extraction from various seeds such as sunflower kernel (Dominguez et al ., 1995), shea tree (Tano-Debrah and Ohta 1994, 1995a), canola, cocoa beans (Tano-Debrah and Ohta 1995b) and coconut (McGlone et al ., 1986; Tano- Debrah and Ohta 1997). The enzymes used in the extraction 64 T. The process that were most frequently referred to in the literature are protease, a amylase, cellulose and pectinase. However, there is no literature on the extraction of oil from papaya seeds using the enzymatic process, even though these seeds have proven to be valuable sources of usable oil (Harvey et al ., 1978). Thus, the aim of this work is to determine and to compare the physicochemical properties and the quality of oil extracted from papaya seeds using different enzymes with that of oil extracted using solvents. MATERIALS AND METHODS

PAPAYA FRUIT

Whole papaya fruits were procured from Jubilee market in Kisumu. Most of the chemicals used in this investigation were of analytical grade. The equipments were obtained from Department of Chemistry and Botany, Maseno University.

PRETREATMENT OF FRUITS

Procured Papaya were washed, wiped and then stored at 100°C in the cold chamber. Preparation of essential oil For solvent extraction, 150 g of ground seeds were placed in a cellulose paper cone and extracted using light Ethanol in a 5-L Soxhlet extractor for 8 h (AOAC

1984). The oil was recovered by evaporating the solvent using a rotary evaporator Model N-1 (Eyela, Tokyo Rikakikai Company, Ltd, Tokyo, Japan) and the residual solvent was removed by drying in an oven at 60C for 1 h and by flushing with 99.9% nitrogen.

Process Flow Chart of Characteristics of papaya seed oil by (solvent extraction)

Ripe papaya fruit

Cut into two longitudinal halves

Seeds removing manually & Wt of sample

Oven drying 60°C for 24 hrs & Wt of sample

Weigh pre dried extract beaker

Place thimble in glass holding tube attached condenser

Place in hydrous ethyl ether in extract beaker

Put beaker on heater apparatus

Extract for 4 hrs & cooling

Remove extraction beaker and let’s air dried oven night (100°C for 30 min

DISCUSSION

It is seen from table no 1 that the seed contained moisture (7.3%), oil (30.9%), protein (28.1%), ash (8.2%), carbohydrate (25.6%), and crude fibre (19.1%). The result of the present investigation are more or less similar to that of Marfo et al ., (1986) and Pungasari et al (2004).

The chemical properties are the most important characteristics of oil, the FFA and iodine value is measure of the degree of unsaturation of the oil.The data pertaining to the Characteristics of papaya seed oil is reported in Table 2. It is observed from the table 5 that papaya seed oil consist of iodine value (65.5), saponification value (155.50), % Unsaponifiable matter (1.37%), and FFA (as % of oleic acid) 0.32. The Characteristics of papaya seed oil are in close agreement with that of Pungasari et al , (2004).

The fatty acid composition of papaya seed oil is shown in Table 3. The result showed that the major fatty acid, the relevant extracted oil were oleic (72.5%) followed by Palmitic (13.5%), stearic acid (4.5%) and Linoleic (2.9%). The results of present investigation are well comparable with the result reported by Pungasari et al , (2004).

Fig: 1.Flow Chart of Papaya Extraction Method

CONCLUSION

Percent seed oil was recorded 30.1 and chemical composition of papaya seed was found to be protein (28.1%), Ash (8.2%), Crude fiber (19.1%) and total carbohydrate (25.6%).The papaya seed oil consists of iodine value (65.5), saponification value (155.5), unsaponifiable matter (1.37%) and free fatty acid (0.32%).The major fatty acid of papaya seed oil were oleic acid(72.5%) followed by palmitic acid (13.5%) and stearic acid (4.5%).

REFERENCES

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Table 1:- Chemical composition of carica papaya L . (carica) seeds

* mean of triplicate determination.

Table 2:- Relative fatty acid composition (%) of papaya seed oil

* mean of triplicate determination

CONFLICTS OF INTEREST

“The authors declare no conflict of interest”.

© 2018 by the authors; Published by SKY FOX Publishing Group, Tamilnadu, India. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/ ).