Research Article

High Performance Liquid Chromatography pigments formation of microalgae growth during the development of Pseudo-nitzschia spp. of Cyanobacteria

Martin Rodriguez*, Karen H. Meyer **, Christian Moldaenke **, Dau *** and Chris Scholin *

Published 09/30/2016 .
Email: martinrod@hotmail.com




Author Affiliation

*Centro de Investigacions Marinas, Conselleria de Pesca, Xunta de Galicia, Apdo.13, E-36620 Vilanova de Arousa, Spain, **Centro de Control da Calidade do Medio Marino, Conselleria de Pesca, Xunta de Galicia, Apdo.E-36600 Vilagarcia de Arousa, Spain. ***Instituto Espanol de Oceanografia, Apdo.1552, E-36200 Vigo, Spain.


Article History

Received: Aug 2016 / Accepted: Sep 2016/ Published: Sep 2016


How to Cite This Article

Rodriguez, M., Meyer, K. H., Moldaenke, C., Dau, & Scholin, C. (2016). High Performance Liquid Chromatography pigments formation of microalgae growth during the development of Pseudo-nitzschia spp. of Cyanobacteria. International Journal of Agricultural and Life sciences, 2(3), 42-45. doi: 10.9379/sf.ijals. 122063-006-0081-x


Copyright

© 2016 Rodriguez et al. This is an open accessarticle distributed under the terms of the CreativeCommons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original author andsource are credited.


Competing Interests

The authors have declared that no competing interests exist.


Abstract

Pseudo-nitzschia blooms from the Ri'a de Pontevedra (NW Spain) were studied by light microscopy and HPLC pigment analysis. Two main Pseudo-nitzschia blooms were registered: the first one in summer had up to 800.000 cells L’ and the second in winter had up to 68.000 cells L'1. During the first bloom amnesic shellfish poisoning (ASP) was not detected and the dominant species was P. fraudulenta. During the second bloom ASP toxicity was detected, and the dominant species was P. australis. Pigment analyses from both blooms showed Chi c2 and Chi c3 as major components of the Chi c family, with Chi c a minor component. Although Chi c3 is usually associated with members of Prymnesiophyceae, Pelagophyceae and Dinophyceae, it has also been detected in Pseudo- nitzschia species as P. fraudulenta, P. delicatissima, P. pungens and P. pseudodelicatissima. However, chi c3 is not present in P. multiseries and P. australis, both able to synthesise domoic acid, the causative agent of ASP. The parallel increase of Chi c3 levels and Pseudo- nitzschia cell numbers (throughout the development of a quasi mono-specific blooms of Pseudo-nitzschia spp) can be used as preliminary information while domoic acid analysis and species identification by EM are performed.