Research Article

Evaluate the antimicrobial activity of Biosurfactant against ESBL producing bacterial isolates

B. Madhumathi1, D. Jegadeeshkumar1, M. Anitha2, N. Kannan2 and Chandrasekaran2

Published 09/30/2017 .

Author Affiliation

1Chromopark, Namakkal, Tamilnadu, India. 2Assistant Professor, Department of Biotechnology, Selvamm Arts and Science College, Namakkal, Tamilnadu, India.

Article History

Sep 2017 / Accepted: Sep 2017/ Published: Sep 2017

How to Cite This Article

Madhumathi, B., Jegadeeshkumar, D., Anitha, M., Kannan, N., & Chandrasekaran. (2017). Evaluate the antimicrobial activity of Biosurfactant against ESBL producing bacterial isolates. Int. J. Agr. Life. Sci, 3(3), 208-213. doi: 10.22573/spg.ijals.017.s12200085


2017 Jegadeeshkumar, D. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing Interests

The authors declare no conflict of interest


In this present study, the biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by blue agar plate, oil spreading test and emulsification index. Among the 15 of them, the best isolate was identified as B.cereus based on microscopic and biochemical analysis. Our aim of the present study was to evaluate the antimicrobial activity of biosurfactant against ESBL producing P.aeruginosa isolates. Susceptibility tests were performed by the agar well diffusion method. Among the 5 ESBL isolates, 3 of them were inhibited by biosurfactant. The zone of inhibition ranged from 12 mm to 22 mm and MIC was 10mg/ml. This effect is comparable to antibiotics and therefore the future use of this biosurfactant as broad spectrum antibiotics are highly promising.