Skyfox Publishing group skyfox skyfox Agricultural and Lifesciences International journal of Agricultural and Lifesciences xxxx-xxxx 2454-6127 Skyfox Publishing Group Tamilnadu, INDIA 122063 10.9379/sf.ijals. 122063-006-0081-x Perspective High Performance Liquid Chromatography pigments formation of microalgae growth during the development of Pseudo-nitzschia spp. of Cyanobacteria Martin Rodriguez 1 Karen H. Meyer 2 Christian Moldaenke 3 Dau 4 Chris Scholin 5 Centro de Investigacions Marinas, Conselleria de Pesca, Xunta de Galicia, Apdo.13, E-36620 Vilanova de Arousa, Spain Centro de Control da Calidade do Medio Marino, Conselleria de Pesca, Xunta de Galicia, Apdo.E-36600 Vilagarcia de Arousa, Spain Centro de Control da Calidade do Medio Marino, Conselleria de Pesca, Xunta de Galicia, Apdo.E-36600 Vilagarcia de Arousa, Spain Instituto Espanol de Oceanografia, Apdo.1552, E-36200 Vigo, Spain. Centro de Investigacions Marinas, Conselleria de Pesca, Xunta de Galicia, Apdo.13, E-36620 Vilanova de Arousa, Spain

The authors have declared that no competing interests exist.

 * E-mail: 9 2016 30 9 2016 2 3 122063-006-0081-x This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication

Pseudo-nitzschia blooms from the Ri'a de Pontevedra (NW Spain) were studied by light microscopy and HPLC pigment analysis. Two main Pseudo-nitzschia blooms were registered: the first one in summer had up to 800.000 cells L’ and the second in winter had up to 68.000 cells L'1. During the first bloom amnesic shellfish poisoning (ASP) was not detected and the dominant species was P. fraudulenta. During the second bloom ASP toxicity was detected, and the dominant species was P. australis. Pigment analyses from both blooms showed Chi c2 and Chi c3 as major components of the Chi c family, with Chi c\ a minor component. Although Chi c3 is usually associated with members of Prymnesiophyceae, Pelagophyceae and Dinophyceae, it has also been detected in Pseudo- nitzschia species as P. fraudulenta, P. delicatissima, P. pungens and P. pseudodelicatissima. However, chi c3 is not present in P. multiseries and P. australis, both able to synthesise domoic acid, the causative agent of ASP. The parallel increase of Chi c3 levels and Pseudo- nitzschia cell numbers (throughout the development of a quasi mono-specific blooms of Pseudo-nitzschia spp) can be used as preliminary information while domoic acid analysis and species identification by EM are performed.

Pseudo-nitzschia blooms from the Ri'a de Pontevedra (NW Spain) were studied by light microscopy and HPLC pigment analysis. Two main Pseudo-nitzschia blooms were registered: the first one in summer had up to 800.000 cells L’ and the second in winter had up to 68.000 cells L'1. During the first bloom amnesic shellfish poisoning (ASP) was not detected and the dominant species was P. fraudulenta. During the second bloom ASP toxicity was detected, and the dominant species was P. australis. Pigment analyses from both blooms showed Chi c2 and Chi c3 as major components of the Chi c family, with Chi c\ a minor component. Although Chi c3 is usually associated with members of Prymnesiophyceae, Pelagophyceae and Dinophyceae, it has also been detected in Pseudo- nitzschia species as P. fraudulenta, P. delicatissima, P. pungens and P. pseudodelicatissima. However, chi c3 is not present in P. multiseries and P. australis, both able to synthesise domoic acid, the causative agent of ASP. The parallel increase of Chi c3 levels and Pseudo- nitzschia cell numbers (throughout the development of a quasi mono-specific blooms of Pseudo-nitzschia spp) can be used as preliminary information while domoic acid analysis and species identification by EM are performed.

Pseudo-nitzschia, domoic acid, marine cyanobacteria The authors received no specific funding for this work.