Bio reduction of Silver ions using Centella asiatica L and its Bioactivity Investigation

DOI

AUTHOR AFFILIATION

Department of Biochemistry, School of Life Science, J.J college of arts and science, Pudukkottai-622422. Tamilnadu, India. Department of Biochemistry, Bharathidasan University, Thiruchirapalli-620024, Tamilnadu India. Department of Industrial science and Technology, University of malaysia Pahang, Malaysia-26300. Department of Nanoscience and Technology, Bharathiyar University, Coimbatore-641114 Tamilnadu India

ABSTRACT

In the present study to check potential effect of antimicrobial activity and leaf assisted bio reduction of silver ion using Centella asiatica has been studied. The leaves of Centella asiatica are used for antimicrobial activity and biosynthesis of silver nanoparticles. Instead the C. asiatica analysis of phytochemicals revealed the presence of alkaloids, flavanoids, tannins etc., on treatment of aqueous solution of silver nitrate with the leaf extract of c.asiatica the rapid formation of stable silver nanoparticles at high concentration is observed occur. The reduction of the metal ions and stabilization of silver nanoparticle is believed to occur by the terpenoid, flavonoid constituents of extract as well as by reducing sugar ascorbate and protein molecule in the broth. And also the plant extract were analysed antimicrobial activity of gram positive and gram negative bacteria using by agar well method supplementary 10µl, 20µl, 30µl and control which observed of result zone of inhibition. However finally conclude the promoising effect Centella asiatica leaf extract synthesis of silver nanoparticles are necessary to evaluate the positive use as a new bactericidal material.