In vitro screening for salinity and drought stress tolerance in plant growth promoting bacterial strains

DOI

http://doi.org.in/10.9379-sf.ijals-122067-010-0081-x

AUTHOR AFFILIATION

1Department of Biosciences, College of Arts, Science & Humanities (CASH), Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India 2Amity Institute of Microbial Technology (AIMT), Block ‘E-3’, 4th Floor, Amity University Campus, Sector-125, Noida-201313, Gautam Buddha Nagar, UP, India.

ABSTRACT

The present study was designed to elevate the in vitro bacterial mechanisms related to the plant growth promotion and their tolerance for sodium chloride (NaCl) and polyethylene glycol (PEG) in culture media. Total nine bacterial strains were studied for both stress tolerance under varying concentration of NaCl and PEG. Out of them, three bacterial strains namely Pseudomonas simiae AU, P. koreensis AK-1 and Carnobacterium sp. SJ-5 were found tolerate to stress and further used for biochemical characterization of ACC-deaminase, IAA and Pi-solubilization activities under both stresses. All three strains were exhibited equal amount of Pisolubilization at each stress levels. The strain P. simiae AU significantly presented the highest ACC-deaminase activity (81 nmol/mg/h and 73 nmol/mg/h) and IAA activity (41.5 Hg/mL and 39.08 Hg/mL) at 0.4M NaCl and 10% PEG stress respectively.