In vitro screening for salinity and drought stress tolerance in plant growth promoting bacterial strains
Keywords:
polyethylene glycol (PEG), plant growth promotion, stress tolerance, bacterial strainsAbstract
The present study was designed to elevate the in vitro bacterial mechanisms related to the plant growth promotion and their tolerance for sodium chloride (NaCl) and polyethylene glycol (PEG) in culture media. Total nine bacterial strains were studied for both stress tolerance under varying concentration of NaCl and PEG. Out of them, three bacterial strains namely Pseudomonas simiae AU, P. koreensis AK-1 and Carnobacterium sp. SJ-5 were found tolerate to stress and further used for biochemical characterization of ACC-deaminase, IAA and Pi-solubilization activities under both stresses. All three strains were exhibited equal amount of Pisolubilization at each stress levels. The strain P. simiae AU significantly presented the highest ACC-deaminase activity (81 nmol/mg/h and 73 nmol/mg/h) and IAA activity (41.5 Hg/mL and 39.08 Hg/mL) at 0.4M NaCl and 10% PEG stress respectively.
References
Ashraf MA, Asif M, Zaheer A, Malik A, Ali Q, Rasool M (2013) Plant growth promoting rhizobacteria and sustainable agriculture: A review. Afr J Microbiol Res 7(9):704-709
Bashan Y, Kamnev AA, de Bashan LE: A proposal for isolating and testing phosphate-solubilizing bacteria that enhance plant growth. Biol Fertil Soils 2013a, 49: 1-2
Belimov AA, Dodd IC, Hontzeas N, Theobald JC, Safronova VI, Davies WJ (2009) Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grow in drying soil via both local and systemic hormone signaling. New Phytol 181:413-423
Bharti N., Yadav D., Barnawal D., Maji D., Kalra A. (2013) Exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in Bacopa monnieri (L.) Pennell under primary and secondary salt stress. World J. Microbiol. Biotechnol. 29 379–387 10.1007/s11274-012-1192-1
Chakraborty U, Chakraborty B, Dey P, Chakraborty AP (2015) Role of microorganisms in alleviation of abiotic stresses for sustainable agriculture. Chakraborty U, Chakraborty B (eds) Abiotic stresses in crop plants. Doi 10.1079/9781780643731.0232
Choudhary DK (2012) Microbial rescue to plant under habitat-imposed abiotic and biotic stresses. Appl Microbiol Biotechnol 96:1137–1155
Diby P, Sarma YR, Srinivasan V, Anandaraj M (2005) Pseudomonas fluorescens mediated vigour in black pepper (Piper nigrum L.) under greenhouse cultivation. Ann Microbiol 55(3):171–174
Dimkpa CO, Merten D, Svatos A, Buchel G, Kothe E (2009) Metal induced oxidative stress impacting plant growth in contaminated soil is alleviated by microbial siderophores. Soil Biol Biochem 41:154-162
Dodd IC, Belimov AA, Sobeih WY, Safronova VI, Grierson D Davies WJ (2005) Will modifying plant ethylene status improve plant productivity in water limited environments? 4th International Crop Science Congress
Duan J, Müller KM, Charles TC, Vesely S, Glick BR (2009) 1-Aminocyclopropane-1-carboxylate (ACC) deaminase genes in Rhizobia from southern Saskatchewan. Microb Ecol 57:423–436
Egamberdieva D (2009) Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiol Plant 31:861-864
Egamberdiyeva D (2007) The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl Soil Ecol 36:184–189
Glick BR (2012) Plant Growth-Promoting Bacteria: Mechanisms and Applications. Scientifica doi:10.6064/2012/963401
Glick BR, Nascimento FX, Vicente CSL, Barbosa P, Espada M et al., (2013) Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus. Biocontrol 58:427–433
Gordon SA, Weber RP (1951) Colorimetric Estimation of Indoleacetic Acid. Plant Physiol26(1):192-195
Grover M, Ali SkZ, Sandhya V, Rasul A, Venkateswarlu B (2011) Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World J Microbiol Biotechnol 27: 1231–1240
Jain S, Vaishnav A, Kasotia A, Kumari S, Gaur RK, Choudhary DK (2013) Bacteria-induced systemic resistance and growth promotion in Glycine max L. Merrill upon challenge inoculation with Fusarium oxysporum. PNAS, India Biol Sci 83: 561-567
Kasotia A, Varma A, Choudhary DK (2015) Pseudomonas mediated mitigation of salt stress and growth promotion in Glycine max. Agric Res 4(1):31–41
Khan MS, Zaidi A, Wani PA (2006) Role of phosphate-solubilizing microorganisms in sustainable agriculture – a review. Agron Sustain Dev 27:29–43
Kumari S, Vaishnav A, Jain S, Varma A, Choudhary D K (2015) Bacterial-mediated induction of systemic tolerance to salinity with expression of stress alleviating enzymes in soybean (Glycine max L.Merrill).J Plant Growth Regul.10.1007/s00344-015-9490
Kumari S, Vaishnav A, Jain S, Varma A, Choudhary D K (2016) Induced drought tolerance through wild and mutant bacterial strain Pseudomonas simiae in mung bean (Vigna radiata L.). World J Microbiol Biotechnol (2016) 32:4
Nadeem SM, Zahir ZA, Naveed M, Arshad M (2009) Rhizobacteria containing ACC-deaminase confer salt tolerance in maize grown on salt-affected fields. Can. J. Microbiol 55:1302–1309
Nautiyal CS, Srivastava S, Chauhan PS, Seem K, Mishra A, Sopory SK (2013) Plant growth-promoting bacteria Bacillus amyloliquefaciens NBRISN13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress. Plant Physiol Biochem 66:1–9
Penrose DM, Glick BR (2003) Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant 118:10–5
Pinton, R., Varanini, Z., and Nannipieri, P., 2001, The rhizosphere as a site of biochemical interactions among soil components, plants and microorganisms, in: The Rhizosphere. Biochemistry and Organic Substances at the Soil-Plant Interface, Pinton, R., Varanini, Z. and Nannipieri, P., eds., Marcel Dekker, N.Y., pp. 1-17
Rodziewicz P, Swarcewicz B, Chmielewska K, Stobiecki M, Wojakowska A (2014) Influence of abiotic stresses on plant proteome and metabolome changes. Acta Physiol Plant 36: 1–19
Siddikee MA, Glick BR, Chauhan PS, Yim W, Sa T (2011) Enhancement of growth and salt tolerance of red pepper seedlings (Capsicum annuum L.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity. Plant Physiol Biochem 49, 427-434
Singh YP, Nayak AK, Sharma DK, Gautam RK, Singh RK, Singh R, Mishra VK, Paris T, Ismail AM (2014) Farmer’s participatory varietal selection: a sustainable crop improvement approach for the 21stcentury. Agroecol Sustain Food Syst 38:427–444
Singh YP, Mishra VK, Singh S, Sharma DK, Singh D, Singh US, Singh RK, Haefele SM, Ismail AM (2016) Productivity of sodic soils can be enhanced through the use of salt tolerant rice varieties and proper agronomic practices. Field Crop Res 190:82-90
Spaepen S, Dobbelaere S, Croonenborghs A, Vanderleyden J (2008) Effects of Azospirillumbrasilenseindole-3-acetic acid production on inoculated wheat plants. Plant Soil 312:15–23
Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 31 425–448
Tilak KVBR, Ranganayaki N, Pal KK, De R, Saxena AK, Nautiyal CS, Mittal S, Tripathi AK, Johri BN (2005) Diversity of plant growth and soil health supporting bacteria. Curr Sci 89:136-150
Vaishnav A, Jain S, Kasotia A, Kumari S, Gaur RK, Choudhary DK (2013) Effect of nitric oxide signaling in bacterial treated soybean plant under salt stress. Arch Microboil 195:571–577
Yang J, Kloepper JW, Ryu CM (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci 14:1-4
Zahir ZA, Ghani U, Naveed M, Nadeem SM, Asghar HN (2009) Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC deaminase for improving growth and yield of wheat ( Triticum aestivum L.) under salt-stressed conditions. Arch Microbiol 191:415–424
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