Bio reduction of Silver ions using Centella asiatica L and its Bioactivity Investigation

Authors

  • Palaniselvam, K Department of Biochemistry, School of Life Science, J.J college of arts and science, Pudukkottai-622422. Tamilnadu, India
  • Antony joseph velanganni, A Department of Biochemistry, Bharathidasan University, Thiruchirapalli-620024, Tamilnadu India.
  • Natanamurugaraj Govindan, S Department of Industrial science and Technology, University of malaysia Pahang, Malaysia-26300.
  • Karthi Department of Nanoscience and Technology, Bharathiyar University, Coimbatore-641114 Tamilnadu India

Keywords:

Centella asiatica, Antibacterial, Silver nitrate, bio reduction, silver nanoparticles, Centella asiatica, Antibacterial, bio reduction, silver nanoparticles

Abstract

In the present study to check potential effect of antimicrobial activity and leaf assisted bio reduction of silver ion using Centella asiatica has been studied. The leaves of Centella asiatica are used for antimicrobial activity and biosynthesis of silver nanoparticles. Instead the C. asiatica analysis of phytochemicals revealed the presence of alkaloids, flavanoids, tannins etc., on treatment of aqueous solution of silver nitrate with the leaf extract of c.asiatica the rapid formation of stable silver nanoparticles at high concentration is observed occur. The reduction of the metal ions and stabilization of silver nanoparticle is believed to occur by the terpenoid, flavonoid constituents of extract as well as by reducing sugar ascorbate and protein molecule in the broth. And also the plant extract were analysed antimicrobial activity of gram positive and gram negative bacteria using by agar well method supplementary 10µl, 20µl, 30µl and control which observed of result zone of inhibition. However finally conclude the promoising effect Centella asiatica leaf extract synthesis of silver nanoparticles are necessary to evaluate the positive use as a new bactericidal material.

References

Sharma KN, Tiwari V, Singh BD 2000. Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tiss Org, 63:179-185.

Chakraborty T, Sinha BS, Sukul NC., 1996. Preliminary evidence of antifilarial effect of Centella asiatica on canine dirofilariasis. Fitoterapia, 67:110-112.

Chandran SP., Chaudhary.M., M.Pasricha R., 2006. Synthesis of gold nano triangle and silver nano particles using Aloe vera plant extract. J. Biotechnol. Prog. (2) 537-543.

Elumalai E.K., Prasad, T.N.V.K.V., Hema Chandran J., Viviyan Therasa S., Thirumali T., and David E., 2010. Extra cellular synthesis of silver nanoparticle using leaves of Euphorbia hirta and their antibacterial activities. J.Pharm. Sci S. Res. Vol 2(9) : 549-554.

Govindaraju K., Tamil Selvans, Kiruthiga V., and singaravelu G., 2010. Biogenic silver nanoparticle by solanum turvum and their promising antimicrobial activity. Journal of Biopesticides (3) (1 special issue) 394-399.

Govindaraju, K., Kiruthiga, V., Ganesh Kumar, V. And Singaravelu, G., 2009. Extracellular synthesis of silver nanoparticles by a marine alga, Sargassum wightii Grevilli and their antibacterial effects. Journal of Nanoscience Nanotechnology, 9: 5497-5501.

Huang,J., Li, Q., Sun, D., Lu,Y., Su,Y., Yang, X., Wang, H., Wang, Y., Shau, W., He, N., Hong, J. and Chen,C. 2007. Biosynthesis of silver and gold nanoparticles by novel sun dr eid Cinnamomum camphora leaf. Nanotechnology, 18: 1-11.

les Panacek., Libor Kvýtek., Robert Prucek., Milan Kolar., Renata Vecerova., Nadezda Pizurova., Virender, K. Sharma., Tat¢jana Nevecna and Radek Zboril., 2006. Silver Colloid Nanoparticles: Synthesis, Characterization, and their Antibacterial Activity. Journal of Physical Chemistry B, 110: 16248-16253.

Lowry OH., Rosebrough N.J., Farr A.L., and Randall RJ 1951. Protein measurement with Folin Phenlo Reagent. Journal of Biochemistry Vol (193) : 265-275.

Mukherjee,P., Roy, M., Mandal, B., Dey, G., Mukherjee , P., Ghatak , J.,2008. Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum.Nanotechnology., 19, 75103.

Nair B., and Pradep T., 2002. Coatescence of nano clusters and formation of sub micron crystallites assisted by lacto bacillus strains. Journals of nanotechnology (2): 293-298.

Panacek A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N., 2006. Silver colloid nanoparticles: Synthesis, characterization, and their antibacterial activity. J Phys Chem B;110 (33):16248-16253.

Raveendran P, Fu J, Wallen SL., 2006. A simple and ‘‘green’’ method for the synthesis of Au, Ag, and Au-Ag alloy nanoparticles. Green Chem, 8: 34-38.

Sandi Salope K, and Sandi B., Silver 2004. Nanoparticles as antimicrobial agents, a case study on E-coli as a model for gram negative bacteria. Journal of Colloid and interface science vol (275): 177-182.

Sastry, M., Ahmad, A., Khan, M.I., and Kumar, R., 2004. Microbial nanoparticle production, in Nanobiotechnology, ed. by Niemeyer CM and Mirkin CA. Wiley-VCH, Weinheim.,2004, 126.

Shankar SS, Rai A, Ahmad A, Sastry M., 2004. Rapid synthesis of Au, Ag and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci; 275(2):496-502.

Singaravelu, G., Arockiyamari, J., Ganesh Kumar,V. And Govindaraju, K. 2007. A novel extra cellular biosynthesis of monodisperse gold nanoparticles using marine algae, Sargassum wightii Greville. Colloids and Surfaces B: Biointerfaces, 57:97-101

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Published

19.06.2015

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Research Article

How to Cite

Bio reduction of Silver ions using Centella asiatica L and its Bioactivity Investigation. (2015). International Journal of Agricultural and Life Sciences, 1(1), 14-18. https://skyfox.co/ijals/index.php/als/article/view/4

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