In-vitro antioxidant and GC-MS analysis ethanolic extract of poly herbal drug

Authors

  • Pattabiraman, K Department of SiddhaMedicine, Faculty of Science, Tamil University, Thanjavur- 613010.
  • Muthukumaran, P Department of SiddhaMedicine, Faculty of Science, Tamil University, Thanjavur- 613010

Keywords:

Antioxidant, Free Radicals, GC-MS, Poly herbal Drug

Abstract

Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants due to safe therapeutics. Poly herbal drugs is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism of pharmacological actions, antioxidant properties of the Poly herbal drugs extract were tested using standard in vitro models. The ethanolic extract of Poly herbal drugs exhibited strong scavenging effect on superoxide, nitric oxide radical and reducing power radical scavenging assay. The free radical scavenging effect of Poly herbal drugs extract was comparable with that of the reference antioxidants. The data obtained in the present study suggests that the extract of Poly herbal drugs have potent Invitro antioxidant and Anti Diabetic activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.

References

Auudy, B., Ferreira, F., Blasina, L., Lafon, F., Arredondo, F., Dajas, R. and Tripathi, P.C. (2003). Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J. Ethnopharmacol. 84: 131-138.

Burits M, Bucar F(2000). Antioxidant activity of Nigella sativa essential oil. Phytother Res. 14(5):323-8.

Cao, G., E. Sofic, and R.L. Prior. 1996. Antioxidant capacity of tea and common vegetables. J. Agr. Food Chem. 44:3426–3431.

Chen, C.W. and Ho, C.T. (1995). Antioxidant properties of polyphenols extracted from green and black tea. J. Food lipids. 2: 35-46.

Dinis TCP, Madeira VMC, Almeida MLM (1994). Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161-169

Duran Reges G., Pascoe Lira D., Vilar Rojas C., Medina Navarro R(2004). Diabetogenic effect of STZ diminishes with loss of Nitric oxide role of ultra violet and carboxy PT10 pharmacology, 71(1);17-24.

Ghorbani, A.; Rakhshandeh, H (2012) The most effective herbs for diabetes. Mashhad: Mashhad University of Medical Sciences Publisher, p.21-127.

Gowdhami T, Rajalakshmi AK , Sugumar N , Valliappan REvaluation of antimicrobial activity of different solvent extracts of aromatic plant: Jasminum sambac linn. Int J Res Pharm Sci 2015, 5(4) ; 18 –23.

Hashem, F.A. (2007) Investigation of free radical scavenging activity by ESR for coumarins isolated from Tecoma radicans. J.Med.Sci. 7:1027-1032.

Huie RE, Padmaja S. (1993).The reaction of no with superoxide. Free Radic Res Commun. 18(4):195-9.

Jose J.K. and Kuttan R (2000) Hepatoprotective activity of Emblica officinalis and Chyavanprash. J Ethnopharmacol. 72, p.135.

Kalimuthu K. & Prabakaran R. (2013). Preliminary Phytochemical Screening and Gc-Ms Analysis Of Methanol Extract Of Ceropegia Pusilla.International Journal Of Research In Applied, Natural And Social Sciences (Impact: Ijranss) Vol. 1, Issue 3, 49-58.

Karthikeyan R., Srinivasan K., Muthukumaran P., Prithiviraj P., Sadhana R & Kumaravel S (2015). Organochlorine and Organophosphorus Pesticide Residues analysis by GC-MS/MS in Raw Cow Milk. In International Journal of Agricultural and Life sciences 1 (4), pp. 7–11. Available online at 10.9379/sfjal-122059-002-0081-x.

Koleva, I.I, Van Beek, T.A., Linssen, J.P.H., De Groot, A., Evstatieva, L.N., (2002): Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13: 8-17.

Komal Kumar J and Devi Prasad AG (2011). Identification and comparison of biomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans by using FTIR. Romanian J. Biophysics; 21(1):63-71.

Kunchandy. E & Rado M.N.A (1990).Oxygen radical scavenging activity of curcuminoid. Int J Pharmacognosy, 58, p. 237.

Lalitharani S., Mohan V.R., Regini G.S., Kalidass C (2009) GC-MS analysis of ethanolic extract of Pothos scandens L. leaf. J. Herb. Medi. Toxicology. 3: 159-160.

Merlin N. J., Parthasarathy V., Manavalan R and Kumaravel S.(2009)Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS. Pharmacognosy Research., 1(3), 152-156.

Moncada S., Palmer R.M. and Higgs E.A. (1991). Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological Reviews 43: 109–142.

Qureshi, R. G.R. Bhatti and R. A. Memon (2010). Ethnomedicinal uses of herbs from Northern part of Nara Desert, Pakistan. Pak. J. Bot., 42(2): 839-851.

Re R, Pellegrini N., Proteggente, A., Pannala, A., Yang, M, Rice_Evans, C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic Biol Med., 26: 1231-7.

Sharma S, Mustafiz A, Singla-Pareek S, Shankar Srivastava P, Sopory S (2012) Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice. Plant Signal Behav , 7(10):1337-45.

Shimada K, Fujikawa K, Yahara K, Nakamura T (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem., 40: 945-948.

Sreejayan Rao M. N. A.(1997) J. Pharm. Pharmacol., 49, 105.

Taylor G, Bagby R, Parker J. (1997) Disorders of affect regulation. Cambridge: Cambridge University Press.

Yermilov V., Rubio J., Becchi M., Friesen M.D., Pignatelli B. and Ohshima H. (1995). Formation of 8- nitroguanine by the reaction of guanine with peroxynitrite in vitro. Carcinogenesis 16: 2045–2050.

Yokozawa, T, Chen C. P, Dong E, Tanaka T, Nonaka G. I, Nishioka I., (1998) Biochemical Pharmacology., 56, 213.

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Published

30.03.2017

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Research Article

How to Cite

In-vitro antioxidant and GC-MS analysis ethanolic extract of poly herbal drug. (2017). International Journal of Agricultural and Life Sciences, 3(1), 106-114. https://skyfox.co/ijals/index.php/als/article/view/23

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